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应用放射免疫法诊断寄生虫病,需用同位素标记各种寄生虫抗原。无论用氯胺-T法或过氧化物酶法标记,同位素碘主要被结合在酪氨酸苯环的羟基上;此外,碘还可以被取代在组氨酸的咪唑基或半胱氨酸的巯基上。为了解常用寄生虫抗原的氨基酸组成,我们对血吸虫虫卵抗原及血吸虫、肺吸虫、肝吸虫的成虫抗原作了氨基酸测定。
Application of radioimmunoassay diagnosis of parasitic diseases, the need for isotope labeling of various parasite antigens. Whether labeled with the chloramine-T method or the peroxidase method, the isotope iodine is mainly bound to the tyrosine benzene ring hydroxyl; In addition, iodine can also be substituted in histidine imidazole or cysteine Thiol. In order to understand the amino acid composition of commonly used parasite antigens, we made amino acid determinations against Schistosoma japonicum egg antigens and the adult antigens of schistosomiasis, paragonimiasis and hepatic flukes.