论文部分内容阅读
本文报道了以２－（６－甲基－２－苯并噻唑偶氮）－５－二乙氨基酚（ＭＢＴＡＥ）作柱前衍生化试剂，在ＳｐｈｅｒｉｓｏｒｂＣ＿８柱上，以甲醇：水＝８０：２０（Ｖ／Ｖ），含２ｍｍｏｌ／ＬＬｉ＿２ＳＯ＿４和１０ｍｍｏｌ／ＬｐＨ５．０醋酸盐缓冲溶液作流动相，反相高效液相色谱分离和测定了Ｆｅ￣（２＋）、Ｃｏ￣（２＋）、Ｎｉ￣（２＋）和Ｃｕ￣（２＋）。各金属离子的检出限（Ｓ／Ｎ＝３）分别为（ｐｇ）：Ｆｅ￣（２＋）６．４０，Ｃｏ￣（２－）１．３８，Ｎｉ￣（２＋）４．４７，Ｃｕ￣（２＋）７．２８。方法用于欧洲标准局，黑麦草标样的分析，所得结果与标准值相符。
In this paper, a pre-column derivatization reagent with 2- (6-methyl-2-benzothiazolylazo) -5-diethylaminophenol (MBTAE) was reported on a Spherisorb C8 column with methanol: water = 80:20 (2 +), Co ~ (2 +) and Ni ~ (2 +) were detected by reversed-phase high performance liquid chromatography with a mobile phase containing 2mmol / L Li_2SO_4 and 10mmol / L pH5.0 acetate buffer solution. 2+) and Cu ~ (2+). The detection limits (S / N = 3) of each metal ion were (pg): Fe ~ (2+) 6.40, Co ~ (2-) ~ 1.38, Ni ~ (2+) ~ 4.47, Cu ~ (2+) 7.28. The method was applied to the analysis of the standards of the European Bureau of Standards and ryegrass, and the results were consistent with the standard values.