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目的 :研究慢性上颌窦炎患者耐甲氧西林金黄色葡萄球菌 (MRSA)的分布和对常见抗菌药物的耐药情况。方法 :采用多重聚合酶链反应 (PCR)同时检测金葡菌的耐药基因 mec A及辅助基因 fem A ;药敏试验采用纸片扩散法。结果 :fem A基因为金葡菌的特有基因 ,mec A基因在 MRSA中检出率为 96 .2 % (5 1/ 5 3)。 MRSA除对万古霉素敏感外 ,对其它抗菌药物都有耐药。结论 :多重 PCR能快速而准确地鉴定 MRSA;MRSA对多种抗菌药物有耐药性。
Objective: To study the distribution of methicillin-resistant Staphylococcus aureus (MRSA) and the resistance to common antibiotics in patients with chronic maxillary sinusitis. Methods: Multiplex polymerase chain reaction (PCR) was used to detect the drug resistance gene mec A and fem A of S. aureus simultaneously. The susceptibility test was performed by disk diffusion method. Results: The fem A gene was a specific gene of S.aureus. The detection rate of mec A gene in MRSA was 96.2% (51/53). In addition to being sensitive to vancomycin, MRSA is resistant to other antibacterials. Conclusion: Multiplex PCR can rapidly and accurately identify MRSA. MRSA is resistant to various antimicrobial agents.