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目的观察Nogo-p4对大鼠脊髓来源神经干细胞(NSCs)分化形成双极形星形胶质细胞突起长度的影响。方法取4只出生24h内的Wistar大鼠,悬浮培养法培养大鼠脊髓来源的NSCs,形成神经球后,以免疫荧光法鉴定NSCs。把NSCs分为A、B两组,A组加入血清,B组加入血清和Nogo-p4,分化7d后,胶质纤维酸性蛋白(GFAP)抗体标记星形胶质细胞,测量双极形星形胶质细胞突起长度。结果 NSCs分化第7天,A组和B组均可形成双极形星形胶质细胞。B组中双极形星形胶质细胞的突起长度明显短于A组。结论 Nogo-p4可以显著抑制脊髓来源NSCs分化成的双极形星形胶质细胞的突起生长。
Objective To investigate the effect of Nogo-p4 on the length of neurite outgrowth induced by spinal cord-derived neural stem cells (NSCs) differentiated into bipolar astrocytes. Methods Four Wistar rats born within 24 hours after birth were cultured in vitro. Spinal cord-derived NSCs were cultured in suspension culture. After forming neurospheres, NSCs were identified by immunofluorescence. NSCs were divided into groups A and B, group A was added to serum, group B was added to serum and Nogo-p4. After differentiation for 7 days, glial fibrillary acidic protein (GFAP) antibody labeled astrocytes, bipolar star Glial cell protrusion length. Results On day 7 of differentiation, bipolar astrocytes were formed in both groups A and B. B group of bipolar astrocytes protruding length was significantly shorter than the A group. Conclusion Nogo-p4 can significantly inhibit the neurite outgrowth of spinal cord-derived bipolar astrocytes.