论文部分内容阅读
AIM: To introduce an animal model of hepatocellularcarcinoma (HCC) in ACI-rats, and to evaluate the therapeuticeffects of Poly-lactide-co-glycolide(Plcg)-microspheres in thetransarterial chemoembolization (TACE) in this model, aswell the value of this model in the experiments ofinterventional therapy.METHODS: Subcapsular implantation of a solid MorrisHepatoma 3 924A (1 mm3) in the livers was carried out in11 male ACI-rats. The tumor volume (V1) was measured bymagnetic resonance imaging (MRⅠ) (13 days afterimplantation). After laparotomy and retrograde placementof catheter into the gastroduodenal artery (14 days afterimplantation), the following protocols of interventionaltreatment were performed: (A) mitomycin C+Poly-lactide-co-glycolide(Plcg)-microspheres (n＝4); (B) 0.9 % NaCl(control group, n＝7). 13 days after these therapies the changeof the tumor volume (V2) was determined by MRI again.RESULTS: The success rate of tumor implantation reachedto 100 %. The mean tumor volume before TACE (V1) were0.082 cm3 in group A and 0.096 cm3 in group B respectively.The mean tumor volume after TACE (V2) were 0.230 cm3 ingroup A and 1.347 cm3 in group B respectively. The meanV2/V1 were 2.860 in group A and 27.120 in group Brespectively. Compared to the control group (group B),groups A showed a significant reduction of tumor growth(P＝0.004) in the period of observation.CONCLUSION: The growth of liver tumor could be obviouslyprevented by utilizing Plcg-mitomycin-microspheres in TACEin animal model. This rat model of HCC is suitable for theexperimental studies of interventional therapy.