肾穿刺活检组织诊断中,PASM(六胺银染色法)染色是重要的检查方法,能清晰地显示基底膜增厚“叮突”、“双轨”等病理变化,通过浸银染色,还可以观察肾小球毛细血管或球囊壁基底膜在炎性损伤(如断裂、增生、折叠等)中的形态学改变。为提高染色质量,我们对传统的方法进行了改良,在实际应用中取得了较好的效果,现将具体方法介绍如下。 In the diagnosis of renal biopsy tissue, PASM staining is an important method to detect the thickening of the basement membrane and pathological changes such as “sting”, “double track” and so on, , Can also observe the glomerular capillary or balloon wall of the basement membrane in inflammatory lesions (such as rupture, proliferation, folding, etc.) in the morphological changes. In order to improve the dyeing quality, we have improved the traditional methods and achieved good results in practical applications. The specific methods are introduced as follows.