论文部分内容阅读
在低硒酵母配制的低硒饲料的基础上，加亚硒酸钠配成含硒量为０．２和５．０ｍｇ／ｋｇ（适硒和高硒）的两组饲料来喂养雄性断乳Ｗｉｓｔａｒ大鼠。在实验２０周末处死大鼠，测定组织器官的细胞内谷胱甘肽过氧化物酶（ｃＧＰＸ）、细胞外谷胱甘肽过氧化物酶（ｅＧＰＸ）、磷脂氢谷胱甘肽过氧化物酶（ＰＨＧＰＸ）、Ⅰ型脱碘酶（ＩＤⅠ）和Ⅱ型脱碘酶（ＩＤⅡ）活性、硒蛋白Ｐ和硒蛋白Ｗ以及组织硒含量，并对所有大鼠的肝脏进行病理学检查。结果发现高硒饲料组大鼠的体重明显低于适硒饲料组。高硒组动物组织中硒含量明显高于适硒组。两组动物肝脏无明显病理学差异，但高硒组大鼠的血浆ｅＧＰＸ活性，肾脏、心脏和睾丸中的ｃＧＰＸ活性，肝、肾和甲状腺的ＩＤⅠ活性，心脏和睾丸中的ＰＨＧＰＸ活性以及大鼠体重明显低于适硒饲料组。提示它们可以作为硒中毒的早期生化指标。
On the basis of low-selenium feed formulated with low-selenium yeast, sodium selenite was dosed into two groups of feed with selenium content of 0.2 and 5.0 mg / kg (suitable selenium and high selenium) to feed male weanling Wistar Rat. Rats were killed at the end of the experiment 20 weeks, and the contents of intracellular glutathione peroxidase (cGPX), extracellular glutathione peroxidase (eGPX), phospholipid hydroperoxide glutathione peroxidase (PHGPX), type Ⅰ deiodinase (IDⅠ) and type Ⅱ deiodinase (IDⅡ) activity, selenoprotein P and selenoprotein W and tissue selenium content. All the rats were pathologically examined for liver. The results showed that the body weight of rats in high-selenium group was significantly lower than that in the group of selenium-fed group. Selenium content in high selenium group was significantly higher than selenium group. There was no obvious pathological difference between the two groups of animals, but plasma eGPX activity, cGPX activity in kidney, heart and testis, IDⅠ activity in liver, kidney and thyroid, PHGPX activity in heart and testis, Weight was significantly lower than the selenium feed group. Suggesting that they can be used as early biochemical indicators of selenium poisoning.