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目的 :探讨用中药海参猴桃液 (SSAP)辅以基因重组人白细胞介素 2 (rIL -2 )体外培养扩增、激活免疫杀伤细胞LAK的机理 ,观察SSAP辅以少量rIL -2能否提高及在多大程度上提高LAK细胞的杀瘤活性。方法 :通过提取健康供血者的外周血单个核细胞作为待培养细胞 ,设空白对照组 (加等量生理盐水 )、阳性对照组 (1 0 0 0U/mlrIL -2 1ml)、实验 1组 (1 0 0 0U/mlrIL -2 0 .5ml +30 0 0 μg/mlSSAP 1ml)、实验 2组 (1 0 0 0U/mlrIL -20 .1ml+30 0 0 μg/mlSSAP 1ml) ,4组在不同条件下培养LAK细胞 ,分别用H -TdR释放法检定其体外对人肝癌BEF -740 4杀伤活性。结果 :30 0 0 μg/ml的SSAP +1 0 0 0U/mlrIL -20 .1ml在培养第 3天便能测出杀伤活性 ,第 9天达峰值 [杀瘤率 (75 .5± 1 3.6 ) % ],杀瘤活性与 1 0倍量rIL -2单独诱导激活的LAK细胞杀瘤活性相近 [杀瘤率 (92 .9± 2 1 .3) % ],两组比较无显著性差异 (P >0 .0 5 ) ,而且杀瘤活性持续 1 8天。结论 :SSAP辅以少量的rIL -2能大量扩增激活LAK细胞 ,增强其对肿瘤细胞的杀伤力 ,更重要的意义在于获得的LAK细胞杀伤力强大而持久 ,同时可减少rIL -2的用量
OBJECTIVE: To investigate the mechanism of the in vitro culture and expansion of activated killer cells (LAKs) with the use of Chinese herbal medicine (SSAP) and recombinant human interleukin 2 (rIL -2 ). And to what extent do they increase the killing activity of LAK cells. METHODS: Peripheral blood mononuclear cells from healthy blood donors were used as the cells to be cultured. The blank control group (with equal volume of normal saline), positive control group (100 U/mlrIL -2 1 ml) and experimental group 1 (1) were used. 0 0 0U/mlrIL -2 0 .5ml +30 0 μg/ml SSAP 1ml), 2nd experiment group (100uU/mlrIL - 20 .1ml + 300g/ml SSAP 1ml), 4 groups under different conditions The LAK cells were cultured and their killing activity against human hepatoma BEF-7404 was assayed by H-TdR release method. RESULTS: The killing activity was measured on the 3rd day of culture at 30.0 μg/ml SSAP +1 00 U/mlrIL - 20.1 ml, peaking on the 9th day [raticidal rate (75. 5± 1 3.6 ) % ], the tumorigenesis activity was similar to the tumorigenesis activity of LAK cell induced by 10 times the amount of rIL-2 alone [the tumor killing rate was 92.9±21.3%]. There was no significant difference between the two groups (P). > 0 . 0 5 ), and the killing activity lasts 18 days. Conclusion: SSAP supplemented with a small amount of rIL-2 can activate and activate LAK cells in large numbers and enhance its lethality to tumor cells. The more important significance is that the obtained LAK cells have a strong and lasting killing effect and can reduce the amount of rIL-2.