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目的探讨微卫星不稳定性(MSI)在胃癌中的表现及其临床诊断价值。方法选择60例通过胃镜标本活检确诊为胃癌的初治患者,另外选择需要胃镜检查的健康成年人20例。实验组患者收集胃液的同时做胃镜活检,对照组只收集胃液。所有标本均进行DNA提取、选择10个微卫星位点银染聚合酶链式反应-简单序列长度多态法(PCR-SSCP)检测MSI,并追踪随访患者20个月。结果胃液沉渣中MSI检出率要高于镜检标本(P<0.05);MSI与肿瘤生物学特性有很好的相关性(P<0.05);随访20个月,胃液沉渣MSI对肿瘤复发有很好的预测性。结论MSI与肿瘤生物学特性有很好的相关性,胃液沉渣MSI检出率要高于镜检标本。
Objective To investigate the manifestations and clinical diagnostic value of microsatellite instability (MSI) in gastric cancer. Methods A total of 60 patients with gastric cancer who had been diagnosed with gastroscopy biopsy were selected. In addition, 20 healthy adults requiring gastroscopy were selected. In the experimental group, gastroscope biopsy was performed while gastric juice was collected. The control group only collected gastric juice. All specimens were extracted with DNA, 10 microsatellite loci were selected and silver staining polymerase chain reaction--simple sequence length polymorphism (PCR-SSCP) was used to detect MSI, and follow-up patients were followed for 20 months. Results The detection rate of MSI in gastric fluid sediment was higher than that of microscopic specimens (P<0.05); MSI had a good correlation with the biological characteristics of tumors (P<0.05); after 20 months of follow-up, gastric fluid sediment MSI had tumor recurrence. Very predictable. Conclusion MSI has a good correlation with the biological characteristics of tumors. The detection rate of MSI in gastric fluid sediments is higher than that of microscopic specimens.