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目的采用HPLC法测定香连金黄软膏中的黄芩苷和姜黄素。方法采用C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.2%磷酸,梯度洗脱,流速1.0 m L·min~(-1),柱温30℃,检测波长分别为280 nm(黄芩苷)和430 nm(姜黄素)。结果黄芩苷0.1020~1.2750μg(r=0.9999)、姜黄素0.0250~0.3125μg(r=0.9999)与峰面积呈良好的线性关系;平均回收率分别为98.93%、99.53%,RSD分别为1.24%、1.32%。结论所用方法简便、灵敏、准确,可用于香连金黄软膏的质量控制。
Objective To determine the baicalin and curcumin in Xianglian golden ointment by HPLC. Methods The mobile phase consisted of acetonitrile - 0.2% phosphoric acid with gradient elution at a flow rate of 1.0 mL · L -1 on a C 18 column (250 mm × 4.6 mm, 5 μm) 280 nm (baicalin) and 430 nm (curcumin) respectively. The results showed that baicalin 0.1020 ~ 1.2750μg (r = 0.9999), curcumin 0.0250 ~ 0.3125μg (r = 0.9999) showed a good linear relationship with the peak area. The average recoveries were 98.93% and 99.53%, respectively, with RSDs of 1.24% 1.32%. Conclusion The method is simple, sensitive and accurate, and can be used for the quality control of Honglian golden ointment.