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为研究以２型登革病毒ＮＳ３基因构建的核酸疫苗能否诱导体液免疫，用表达２型登革病毒ＮＳ３的重组真核表达质粒ＰＳＶ·ＮＳ３直接接种Ｂａｌｂ／ｃ小鼠。结果发现在接种后２周即可检测到ＩｇＭ抗体，该抗体在加强接种后滴度显著升高，６周达最高水平至少维持２个月；ＩｇＧ抗体在接种后４周可检测到，８周达最高水平至少维持２个月。Ｗｅｓｔｅｒｎｂｌｏｔ证实了接种小鼠血清中有ＮＳ３特异性的ＩｇＧ抗体。这些结果表明我们接种的ＰＳＶ·ＮＳ３能诱导Ｂａｌｂ／ｃ小鼠的体液免疫。
To investigate whether humoral immunity can be induced by nucleic acid vaccine constructed with Dengue virus type 2 dengue virus, Balb / c mice were directly inoculated with recombinant eukaryotic expression plasmid PSV-NS3 expressing Dengue virus type 2. As a result, it was found that IgM antibody was detected two weeks after inoculation. The antibody showed a marked increase in titers after boosting, reaching a maximum level of at least 6 months at 6 weeks. IgG antibodies were detected 4 weeks after inoculation and 8 weeks The highest level of at least 2 months. Westernblot confirmed the NS3-specific IgG antibodies in sera of mice vaccinated. These results indicate that we vaccinated PSV · NS3 can induce humoral immunity in Balb / c mice.