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目的筛选分离葛根总黄酮的最佳树脂,并对影响分离的各种因素进行系统的研究,使纯化工艺达到最优。方法采用静态与动态的吸附-解吸两种方法,利用紫外可见分光光度计测量葛根总黄酮的含量,研究不同大孔吸附树脂及其不同的工艺条件对总黄酮分离纯化的影响。结果 SP70分离效果最好,其最佳工艺为药液浓度0.5 g·mL-1(相当于原生药)、pH为5 -6、以2 BV·h-1速率进行上样,上样量为60 BV,以5 BV的70%乙醇、2 BV·h-1的流速进行洗脱,效果最佳。经SP70处理后的葛根总黄酮的含量可达80%以上。结论大孔吸附树脂SP70分离纯化总黄酮效果较好,适合工业生产。
Objective To screen the best resin for the separation of total flavonoids from Pueraria lobata, and systematically study the factors affecting the separation, so as to optimize the purification process. Methods Static and dynamic adsorption-desorption methods were used to measure the content of total flavonoids in Radix Puerariae by ultraviolet-visible spectrophotometer. The effects of different macroporous resin and its different process conditions on the separation and purification of total flavonoids were studied. Results SP70 had the best separation effect. The best process was that the concentration of the drug solution was 0.5 g·mL-1 (corresponding to the original drug), the pH was 5 -6, and the sample was loaded at 2 BV·h-1. 60 BV was eluted with 5 BV of 70% ethanol and 2 BV·h-1 for the best results. The total flavonoid content of Radix Puerariae treated with SP70 can reach more than 80%. Conclusion The macroporous adsorption resin SP70 is effective for the separation and purification of total flavonoids and is suitable for industrial production.