论文部分内容阅读
目的利用稻瘟霉生物活性成分跟踪筛选模型,对海星中皂苷进行提取和分离纯化。方法用65%EtOH对粉碎的海星(干)进行回流提取,减压浓缩后均匀分散于水中,用石油醚和正丁醇进行萃取,浓缩正丁醇得海星总皂苷,对其进行稻瘟霉生物活性测试。海星总皂苷粗提物通过正相硅胶、反相硅胶及葡聚糖凝胶LH-20柱层析进行分离纯化。用薄层层析法(TLC)检测把相同的洗脱液收集在一起,得到所需要的有效部位。结果与结论通过稻瘟霉生物活性筛选知海星乙醇粗提物、海星总皂苷均具有抗有丝分裂活性,而石油醚粗提物没有显著的活性。海星总皂苷通过正相硅胶和反相硅胶柱层析分离得到D1～D66个含有海星皂苷的组分,其中D1、D3、D4、D5、D6在TLC上呈现出单一斑点。D2进一步通过葡聚糖凝胶LH-20柱层析进行分离,得到收率约为81.2%的组分a,收率约为3%的组份b。
Objective To extract and separate the saponins from Starfish by using the tracking model of bioactive components of Pyricularia oryzae. Methods The crushed starfish (dry) was extracted by refluxing with 65% EtOH, concentrated under reduced pressure and then dispersed in water. The extraction was carried out with petroleum ether and n-butanol. The n-butanol was concentrated to obtain the total saponins of starfish, which were used for the production of Magnaporthe grisea. Activity test. The crude extract of starfish total saponins was separated and purified by normal phase silica gel, reversed phase silica gel, and Sephadex LH-20 column chromatography. The same eluent was collected together by thin layer chromatography (TLC) detection to obtain the desired active site. RESULTS AND CONCLUSION: Through the screening of the biological activity of Pyricularia oryzae, the crude ethanol extracts of starfish and starfish have anti-mitotic activity, while the petroleum ether extract has no significant activity. Starfish total saponins were separated by normal phase silica gel and reversed-phase silica gel column chromatography to obtain D1 to D66 fractions containing starfish saponins, among which D1, D3, D4, D5, and D6 showed single spots on TLC. The D2 was further separated by chromatography on a Sephadex LH-20 column to obtain fraction a with a yield of about 81.2% and fraction b with a yield of about 3%.