采用逆转录PCR技术自人胎肝细胞中克隆出人β_2GP1第5功能区cDNA,长度为298bp,经纯化后定向插入表达性载体pGEX-2T,并筛选出带有插入片段的阳性克隆,重组克隆经IPTG诱导,可产生约36kD的融合蛋白,为研究抗磷脂抗体所针对的抗原及表位奠定了基础. The cDNA of human β_2GP1 5’-functional region was cloned from human fetal liver cells by RT-PCR and its length was 298bp. After purification, the cDNA was inserted into expression vector pGEX-2T, and positive clones with inserted fragment were screened. Recombinant clones Induced by IPTG, can produce about 36kD fusion protein, which lays the foundation for the study of anti-phospholipid antibodies against the epitopes and epitopes.