论文部分内容阅读
目的探讨自体骨髓单核细胞(ABM-MNCs)移植至心肌梗死区及周边区后的存活、分化状况,以及对心功能的影响。方法将40只日本大耳雄兔随机分为细胞移植组和对照组各20只。采用结扎左冠状动脉前降支的方法建立急性心肌梗死(AMI)模型。细胞移植组于梗死后第6天于自体髂骨处抽取骨髓,分离得到单核细胞后,以Brdu标记,经心外膜注射到梗死区及周边区。对照组仅注射等量的生理盐水。移植6周后,进行组织学及免疫组织化学检查。结果移植后6周,两组动物的心功能均有改善,但两组间有显著性差异。Brdu标记示移植组梗死区内存在阳性染色的移植细胞,而周边区可见Brdu染色阳性的心肌细胞及血管内皮细胞。免疫荧光染色示梗死区内Brdu染色阳性的移植细胞抗心肌特异性肌动蛋白抗体阴性,而周边区内Brdu染色阳性的移植细胞抗心肌特异性肌动蛋白抗体阳性。对照组未发现Brdu染色阳性的细胞。移植组梗死周边区及远离梗死区的毛血管密度均高于对照组(P<0.05),但梗死区内的毛细血管密度两组间无显著性差异(P>0.05)。结论ABM-MNCs移植至急性梗死心肌后,可在梗死区内及周边区存活,并可在周边区分化为具有心肌功能的细胞及血管内皮细胞,增加梗死周边区的血管密度,改善心功能。
Objective To investigate the survival and differentiation of autologous bone marrow mononuclear cells (ABM-MNCs) transplanted into the myocardial infarction and peripheral areas and their effects on cardiac function. Methods Forty male Japanese big ears were randomly divided into two groups, 20 in each cell transplantation group and control group. Acute myocardial infarction (AMI) model was established by ligating left anterior descending coronary artery. The bone marrow was harvested from the autologous iliac bone on the 6th day after infarction in the cell transplantation group. The mononuclear cells were isolated and labeled with Brdu. The epicardium was injected into the infarct area and peripheral area. The control group was injected with the same amount of saline only. Six weeks after transplantation, histological and immunohistochemical examinations were performed. Results Six weeks after transplantation, heart function of both groups improved, but there was significant difference between the two groups. Brdu labeling showed that there was positive staining of transplanted cells in the infarcted area in the transplantation group, while Brdu-positive cardiomyocytes and endothelial cells were seen in the peripheral area. Immunofluorescence staining showed that Brdu-positive transplanted cells in the infarcted area were negative for anti-cardiac muscle specific actin and that Brdu-positive cells in the peripheral area were positive for anti-myocardial specific actin. Brdu positive cells were not found in the control group. There was no significant difference in capillary density in the infarct area between the two groups (P> 0.05). CONCLUSIONS: ABM-MNCs can survive in the infarct area and surrounding area after transplanted to acute infarcted myocardium, and differentiate into myocardial cells and endothelial cells in the peripheral area, increase the blood vessel density in the peripheral area and improve cardiac function.