目的研究中药奇蒿氯仿、乙酸乙酯提取部位中具有抗菌活性的具体组分。方法采用硅胶柱层析法对奇蒿氯仿、乙酸乙酯提取部位进行组分分离,选用临床常见致病菌通过纸片扩散法和常量肉汤稀释法对分离得到的各组分进行体外药敏试验,通过物理化学方法进一步鉴定活性成分的结构。结果奇蒿氯仿、乙酸乙酯部位的多个分离组分对临床常见致病菌具有不同程度抗菌活性,活性显著的组分经结构鉴定确定为:芹菜素、异泽兰黄素、咖啡酸,其中异泽兰黄素对金黄色葡萄球菌抗菌活性最强,其最小杀菌浓度为0.062 5mg/mL。结论本实验方法简单、快捷,可准确有效地得到奇蒿氯仿、乙酸乙酯活性部位的有效抗菌成分,并首次报道了异泽兰黄素的抗菌活性。 Objective To study the specific components of Chinese herbal medicine Artemisia selengensis with antibacterial activity in chloroform and ethyl acetate extract. Methods Silica gel column chromatography was used to separate the constituents of the extract of Artemisia oeri from chloroform and ethyl acetate. The common pathogenic bacteria were isolated by disk diffusion method and constant broth dilution method. Tests to further identify the structure of the active ingredient by physicochemical methods. Results Several components of Artemisia sp. Chinensis and ethyl acetate fraction had different degrees of antibacterial activity against common pathogenic bacteria in clinical practice. The components with significant activity were confirmed by structure identification as follows: apigenin, isoprenoid, caffeic acid, Among them, isoprenoid had the strongest antibacterial activity against Staphylococcus aureus, and its minimum bactericidal concentration was 0.062 5 mg / mL. Conclusion The experimental method is simple and rapid, and the effective antibacterial components of the active fractions of Artemisia ovatis can be obtained accurately and effectively. The antibacterial activity of different flavans is reported for the first time.