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目的:探讨Smads蛋白家族在慢性哮喘大鼠肺组织中的定位表达及其mRNA变化情况,以反映其在哮喘大鼠气道重建过程的作用。方法:24只SD大鼠分为正常组和模型组各12只。模型组以10%卵蛋白+10%氮氧化铝生理盐水ip致敏,2周后用2%卵蛋白雾化吸入1 min,隔天1次,引喘,共2周后处死。取肺组织切片(HE染色)作形态学观察;荧光免疫组化法测定Smads蛋白家族在肺组织中的定位;RT-PCR测定Smads蛋白家族mRNA的变化情况。结果:HE染色显示模型组有较明显出血,肺泡均有一定程度扩张,有慢性炎细胞浸润;荧光免疫组化法显示Smads蛋白家族均在在支气管壁和肺泡壁上表达;RT-PCR测定显示模型组Smad2 mRNA较正常组高表达(P<0.05);模型组Smad7 mRNA较正常组显著低表达(P<0.05);两组Smad4 mRNA之间无差异。结论:Smads蛋白家族与慢性哮喘关系密切,在正常与哮喘模型中都可见Smads蛋白家族的表达,在哮喘发生过程中,受体型Smad2 mRNA表达增加,抑制型Smad7 mRNA表达下降,说明TGF-β/Smad信号途径可能被活化。
OBJECTIVE: To investigate the localization of Smads protein family in the lung tissue of chronic asthmatic rats and its mRNA expression to reflect its role in airway remodeling in asthmatic rats. Methods: Twenty-four SD rats were divided into normal group and model group with 12 rats each. The model group was sensitized ip with 10% ovalbumin + 10% oxalicotinate saline. After 2 weeks, the model group was inhaled with 2% ovalbumin for 1 min. The lung tissue sections were taken for morphological observation. The localization of Smads protein family in lung tissues was detected by fluorescence immunohistochemistry. The changes of Smads protein family mRNA were detected by RT-PCR. Results: Hematoxylin-eosin staining showed that the model group had obvious hemorrhage, the alveoli all had a certain degree of expansion and infiltration of chronic inflammatory cells. Fluorescence immunohistochemistry showed that the Smads protein family was expressed on the bronchial wall and the alveolar wall. RT-PCR showed The expression of Smad2 mRNA in model group was higher than that in normal group (P <0.05). Smad7 mRNA in model group was significantly lower than that in normal group (P <0.05). There was no difference in Smad4 mRNA between the two groups. Conclusion: The Smads protein family is closely related to chronic asthma. Smads protein family is expressed in both normal and asthmatic models. During asthma, the expression of receptor Smad2 mRNA and the expression of Smad7 mRNA decrease, indicating that TGF-β / Smad signaling pathway may be activated.