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目的 :对人轮状病毒抗原非复制型重组腺病毒诱导黏膜免疫的效果进行初步评价。方法 :用表达轮状病毒VP7、VP6基因的 3株重组腺病毒rvAdG1VP7(G)、rvAdG1VP7和rvAdVP6 ,分别通过灌胃和滴鼻两种途径对BALB C小鼠进行 2次免疫后 ,对肺灌洗液和肺、肠粘膜组织匀浆液中轮状病毒特异性的分泌型IgA(secretoryIgA ,SIgA)和local IgG进行检测。结果 :对肺灌洗液中特异性SIgA进行检测 ,发现滴鼻组的免疫学效果明显优于灌胃组。对肺、肠组织匀浆液中特异性IgA的分析表明 ,灌胃途径刺激异位粘膜组织产生免疫应答的能力较弱。对rvAdVP6滴鼻组小鼠肺灌洗液 (1∶2 0 )特异性local IgG和SI gA的阳转率进行比较 ,发现特异性local IgG的应答水平明显高于特异性SIgA。结论 :重组腺病毒可有效诱导针对轮状病毒的黏膜免疫。此研究为轮状病毒基因工程疫苗的免疫方案、免疫途径及免疫保护作用等的进一步研究奠定了基础。
Objective: To evaluate the effect of human non-replicating recombinant human rotavirus on mucosal immunity induced by recombinant adenovirus. Methods: Three recombinant adenovirus rvAdG1VP7 (G), rvAdG1VP7 and rvAdVP6 expressing VP7 and VP6 genes of rotavirus were used to immunize BALB C mice twice after intragastric and intranasal instillation respectively. Lotion and lung, intestinal mucosa homogenate rotavirus specific secreted IgA (secretoryIgA, SIgA) and local IgG were detected. Results: The specific SIgA in lung lavage fluid was detected, and the immunological effect of intranasal group was better than that of intragastric group. Analysis of specific IgA in lung and intestine homogenates showed that the ability of gavage to stimulate ectopic mucosal tissue to produce immune response is weak. The positive rates of local IgG and SI gA specific for lung lavage fluid (1: 200) in rvAdVP6 intranasal mice were found to be significantly higher than those of specific SIgA. Conclusion: Recombinant adenovirus can effectively induce mucosal immunity against rotavirus. This study laid the foundation for further research on the immunization programs, immunological pathways and immunoprotection of rotavirus genetic engineering vaccines.