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采用 FPLC法通过凝胶色谱柱分离 GM- CSF产品中的各组份 ,同时利用峰面积计算GM- CSF在产品中的含量。应用此方法测定了三批进口基因重组 GM- CSF,GM- CSF含量分别为0 .43、0 .40、0 .42 mg/ ml
The components of GM-CSF product were separated by gel column using FPLC method, and the content of GM-CSF in the product was calculated by using the peak area. Using this method, three batches of imported recombinant GM-CSF and GM-CSF were determined respectively at 0 .43, 0 .40 and 0 .42 mg / ml