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目的探讨鼠重组成骨蛋白1(rOP1)在大鼠肾脏缺血再灌注损伤过程中对氧自由基、细胞凋亡的影响及其对肾脏的保护作用。方法观察静脉应用rOP1250μg/kg体重,大鼠肾脏缺血60min,再灌注24h后,血浆超氧化物歧化酶(SOD)、脂质过氧化物丙二醇(MDA)、内皮素1(ET1)的变化,及其对肾脏细胞凋亡的影响。结果rOP1组和缺血再灌注组血浆SOD分别是(108±20)和(86±11)KNU/L,MDA分别是(8.90±0.49)和(11.70±0.81)μmol/L,ET1分别是(130±39)和(179±45)ng/L,rOP1组SOD水平显著性高于缺血再灌注组(P<0.05),而MDA和ET1水平显著性低于缺血再灌注组(P<0.05)。两组肾脏细胞凋亡指数分别是(5.72±3.08)%和(8.85±3.52)%,rOP1组显著性低于缺血再灌注组(P<0.01)。上述两组肾脏的的Miller’s评分分别是(2.0±0.3)和(3.4±0.5),rOP1组显著低于缺血再灌注组(P<0.01)。结论rOP1减轻氧自由基对肾脏缺血再灌注损伤,降低肾脏细胞凋亡指数,对肾脏缺血再灌注损伤具有保护作用。
Objective To investigate the effects of rop1 on oxygen free radicals and apoptosis in rat kidney during ischemia-reperfusion injury and its protective effects on the kidney. After intravenous observe rOP1250μg / kg body weight, renal ischemia 60min, reperfusion 24h, plasma superoxide dismutase (SOD), lipid peroxide glycol (of MDA), endothelin in 1 (ET1), and And its effects on renal cell apoptosis. Results rOP1 group and the R group is a plasma SOD (108 ± 20) respectively (86 ± 11) KNU / L, MDA were (8.90 ± 0.49) and (11.70 ± 0.81) μmol / L, ET1 were ( 130 ± 39) and (179 ± 45) ng / L respectively. The SOD level in rOP1 group was significantly higher than that in ischemia / reperfusion group (P <0.05), while the levels of MDA and ET1 in ischemia / reperfusion group were significantly lower than those in ischemia / 0.05). The apoptosis index of kidney in the two groups was (5.72 ± 3.08)% and (8.85 ± 3.52)%, respectively. The rOP1 group was significantly lower than that of ischemia-reperfusion group (P <0.01). The Miller’s scores in the two groups were (2.0 ± 0.3) and (3.4 ± 0.5), respectively, and were significantly lower in the rOP1 group than in the ischemia-reperfusion group (P <0.01). Conclusion rOP1 can reduce oxygen free radicals on renal ischemia-reperfusion injury, reduce renal cell apoptosis index, and have a protective effect on renal ischemia-reperfusion injury.