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通过对５个籼稻品种（系）配成的５×５双列杂交后代的花药培养出愈率、分化率和绿苗率的分析表明：（１）出愈率、分化率和绿苗率是可遗传的特性，它们的遗传存在基因的加性效应和非加性效应，出愈率和绿苗率还具有明显的胞质效应；（２）一个具有较高出愈率的亲本或组合，其分化率和绿苗率并不一定高，愈伤组织的诱导过程和分化过程可能是由不同基因控制的；（３）在花培育种中，建议建立花培育种亲本圃，结合常规育种技术指导亲本选配．
The results showed that: (1) The rate of emergence, the rate of differentiation and the rate of green plantlet were the best among the 5 × 5 pairs of indica hybrids. Heritable properties, their genetic existence of gene additive effect and non-additive effect, the culling rate and green shoot rate also have obvious cytoplasmic effects; (2) a higher callus or parental combinations, Its differentiation rate and green shoot rate are not necessarily high, and the induction process and differentiation process of callus may be controlled by different genes. (3) In flower breeding, it is suggested to establish flower nursery parents nursery, combined with conventional breeding techniques Guidance parents optional.