背景:脑海马结构是与学习记忆有关的脑区,一般认为与空间认知活动密切相关。在脑缺血后发生的过氧化应激引发海马区和齿状回DNA损伤和DNA修复能力下降,其学习记忆功能也相应下调。目的:探讨红景天对全脑缺血再灌注大鼠学习记忆功能区域海马区及齿状回核酸表达的影响。设计:随机对照实验。单位:武警医学院中心实验室。对象:实验于2002-04/2004-04在武警医学院中心实验室完成,选用Wistar雄性大鼠60只,随机分为5组,每组12只。①红景天1.2g/(kg·d)剂量组。②红景天0.48g/(kg·d)剂量组。③川芎嗪80mg/(kg·d)对照组。④模型组:按给药组相同体积每日蒸馏水灌胃。⑤假手术组:按给药组相同体积每日蒸馏水灌胃。方法:各组大鼠连续灌胃给药7d,用改良的Pulsinelli4-血管阻断法,复制大鼠急性全脑缺血再灌注损伤模型。其中假手术组不灼烧椎动脉,不夹闭颈总动脉。模型制备后用吖啶橙染色法观察脑海马区和齿状回DNA、RNA的表达变化。主要观察指标:各组大鼠脑海马区及齿状回DNA、RNA的表达。结果:60只大鼠均进入结果分析。假手术组DNA和RNA分布均匀,荧光反射边界清晰,反应强度较强;模型组的DNA和RNA荧光反射边界不清,反应强度明显减弱;红景天1.2g/(kg·d)剂量组的DNA和RNA分布均匀,荧光反射边界清晰,反应强度明显增强,与假手术组及川芎嗪80mg/(kg·d)对照组表达分布基本相同。红景天0.48g/(kg·d)剂量组荧光反应强度及分布与模型组比较无明显改变。结论:手术组DNA和RNA荧光反射不清,可能与脑缺血再灌流损伤中氧化应激引起DNA链断裂有关。给予红景天1.2g/(kg·d)剂量大鼠脑海马区及齿状回DNA和RNA荧光反射边界清晰,表明红景天能够抑制氧化应激引起的DNA链断裂,对全脑缺血再灌注大鼠学习记忆功能区域海马区及齿状回具有保护作用。 Background: Brain hippocampal formation is a brain area associated with learning and memory. It is generally believed that the brain is closely related to spatial cognitive activity. The post-cerebral ischemia-induced peroxidative stress induces DNA damage and DNA repair in hippocampus and dentate gyrus, and its learning and memory function is also down-regulated. Objective: To investigate the effect of Rhodiola on the expression of nucleic acid in hippocampus and dentate gyrus of rats with global cerebral ischemia reperfusion. Design: Randomized controlled trials. Unit: Central Laboratory of Armed Police Medical School. PARTICIPANTS: The experiment was performed at the Central Laboratory of the Armed Police Medical College from April 2002 to April 2004. A total of 60 male Wistar rats were randomly divided into 5 groups of 12 in each group. 1 Rhodiola 1.2g/(kg·d) dose group. 2 Rhodiola 0.48g/(kg·d) dose group. 3 Tetramethylpyrazine 80 mg/(kg·d) control group. 4 Model group: Daily distilled water was given in the same volume as the administration group. 5 sham group: daily distilled water was given in the same volume as the administration group. METHODS: Rats in each group were continuously intragastrically administered for 7 days. The rat model of acute global cerebral ischemia-reperfusion injury was duplicated with a modified Pulsinelli 4-vessel occlusion method. The sham-operated group did not burn the vertebral artery and did not clamp the common carotid artery. After the model was prepared, the expression of DNA and RNA in hippocampus and dentate gyrus was observed by acridine orange staining. MAIN OUTCOME MEASURES: Expression of DNA and RNA in hippocampus and dentate gyrus of rats in each group. Results: All 60 rats were involved in the analysis of the results. In the sham operation group, the DNA and RNA were evenly distributed, the fluorescence reflection boundary was clear, and the reaction intensity was strong. The fluorescence and reflection boundaries of the DNA and RNA in the model group were unclear, and the reaction intensity was significantly reduced; Rhodiola 1.2g/(kg·d) dose group The distribution of DNA and RNA was even, the fluorescence reflection boundary was clear, and the reaction intensity was significantly enhanced. The expression distribution was similar to that of the sham operation group and the 80 mg/(kg·d) control group of ligustrazine. There was no significant change in the fluorescence intensity and distribution of Rhodiola rosea 0.48g/(kg·d) dose group compared with the model group. Conclusion: The unclear reflection of DNA and RNA fluorescence in the surgical group may be related to the oxidative stress-induced DNA strand break in cerebral ischemia-reperfusion injury. The fluorescence and reflection boundaries of DNA and RNA in the hippocampus and dentate gyrus of rats given Rhodiola 1.2g/(kg·d) were clear, indicating that Rhodiola can inhibit the DNA strand break caused by oxidative stress, and the global cerebral ischemia Reperfusion of hippocampal region and dentate gyrus in learning and memory regions of rats has a protective effect.