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用高锰酸钾-锇酸固定法制备了5种培养细胞整装内质网标本,并在扫描电镜下对其三维结构进行了观察。观察结果表明内质网是由膜性小管构成的贯穿整个细胞质的管囊网络样膜性区室,并以多种形态深入到细胞伪足及突起中;细胞质中内质网则表现为簇状网络(见于GCM3T3细胞)、多态性多孔扁囊样网络、筛网状网络、条索状网络、大孔条索网状和细孔扁囊样分区网络、不规则管网状和多孔管囊分区网络(见于CV-1细胞)、细管网络(见于CCL187和CCL229细胞)、球囊网络(见于CCL187和A431细胞)和不规则管网状网络(见于A431细胞)等。内质网的这种多态性提示它是一种高度可变的结构,其可变性可能与细胞特性、分化程度、细胞功能状态及细胞骨架系统的分布变化等因素有关。
Five kinds of cultured endoplasmic reticulum samples were prepared by potassium permanganate-osmic acid fixation method. The three-dimensional structure of the samples was observed under scanning electron microscope. The results showed that the endoplasmic reticulum is composed of membranous tubules throughout the cytoplasm of the cystic network-like membranous compartment, and in various forms into the cell pseudopods and protuberances; cytoplasmic endoplasmic reticulum showed tufted Network (found in GCM3T3 cells), polymorphic porous sachet-like networks, mesh-like networks, bar-like networks, macroporous cords and gingival-like network, irregularly tubular networks and porous cells (See CCL 187 and CCL229 cells), balloon network (see CCL 187 and A431 cells) and irregular tube network (see A431 cells) and so on. This polymorphism of the endoplasmic reticulum suggests that it is a highly variable structure, and its variability may be related to cell characteristics, differentiation, cell function status and the distribution of the cytoskeleton system and other factors.