对棉铃虫单核衣壳核多角体病毒 (HaSNPV)的组织蛋白酶 (ν cath)基因进行了序列分析 ,此基因编码区长 10 98bp ,预计编码一个 36 6个氨基酸的蛋白产物。序列分析表明 ,HaSNPV的V CATH蛋白与其它杆状病毒的同源蛋白具有相似的保守结构并保留有相同的酶活性位点 ,根据已知的杆状病毒组织蛋白酶序列构建了ν cath基因的进化树 ,发现HaSNPV的ν cath位于NPV组中一个单独的分枝 ,结合ν cath基因在棉铃虫病毒基因组中的位置与其它NPV有较大不同 ,推测HaSNPV的ν cath基因可能拥有特殊的进化历程。 The sequence of the cathepsin (ν cath) gene of the single-nucleocapsid nuclear polyhedrosis virus (HaSNPV) of Helicoverpa armigera was sequenced. The coding region of this gene is 10 98 bp in length and is expected to encode a protein product of 36 6 amino acids. Sequence analysis showed that the VATH protein of HaSNPV shared similar conserved structure with homologous proteins of other baculoviruses and retained the same enzyme active site. The evolution of ν cath gene was constructed according to the known baculovirus cathepsin sequence Tree. It was found that ν cath of HaSNPV was located in a single branch of NPV group. The location of ν cath gene in the genome of Helicoverpa armigera was different from other NPV. It is speculated that the ν cath gene of HaSNPV may have a special evolutionary history.