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目的：研究Ｔ细胞受体（ＴＣＲ）Ｖβ基因亚家族在免疫后的表达特征。方法：运用ＲＴ－ＰＣＲ及Ｓｏｕｔｈｅｒｎ ｂｌｏｔ技术对乙肝疫苗免疫前后健康成年人ＴＣＲ Ｖβ１－２０基因亚家族的表达水平迸行了半定量研究。结果：Ｖβ６、Ｖβ１４基因亚家族的表达增高；ＶＢ４、ＶＢ９基因亚家族的表达下调。结论：免疫后诱导产生了ＨＢｓＡｇ特异的Ｔ细胞应答，该结果为制定预防和治疗ＨＢＶ感染的免疫策略提供了新的依据。
Objective: To study the expression of Vβ gene subfamily of T cell receptor (TCR) after immunization. Methods: The expression of Vβ1-20 gene subfamily of TCR in healthy adults before and after hepatitis B vaccine immunization was semi-quantitatively studied by RT-PCR and Southern blot. Results: The expressions of Vβ6 and Vβ14 subfamilies were increased, while those of VB4 and VB9 genes were down-regulated. Conclusion: HBsAg-specific T cell responses induced after immunization provide a new basis for the development of immunization strategies for the prevention and treatment of HBV infection.