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原发性肝癌是临床上最常见的恶性肿瘤之一,目前仍在寻找有效的治疗手段.白藜芦醇苷可抑制肺癌细胞以及大肠癌细胞的增殖,但其在肝癌中的作用及具体作用机制并不清楚.本文探讨白藜芦醇苷对大鼠肝癌是否具有预防作用,及其对肝癌细胞系增殖和侵袭的影响.构建大鼠原发性肝癌模型,将其分为正常组、模型组及白藜芦醇苷预防组.病理检测结果显示,与模型组相比,白藜芦醇苷预防组的肝癌发生率明显降低.检测肝组织中microRNA-21表达情况,结果显示,白藜芦醇苷预防组肝中microRNA-21表达明显降低,并且microRNA-21的靶基因PTEN表达上调.在肝癌细胞系SMMC7721和Hep G2中加入白藜芦醇苷,细胞增殖及侵袭能力明显下降,同时伴随microRNA-21表达降低,PTEN表达升高.这提示,白藜芦醇苷可能通过抑制microRNA-21的表达,抑制肝癌的发生,本文结果为预防原发性肝癌提供了新的理论依据,但其临床疗效还需要进一步验证.
Primary liver cancer is one of the most common malignant tumors in clinical practice, and it is still looking for effective treatment.Peridin can inhibit the proliferation of lung cancer cells and colorectal cancer cells, but its role in liver cancer and its specific role Mechanism is not clear.This article discusses whether piceid on rat hepatocarcinoma has preventive effect and its effect on proliferation and invasion of hepatocellular carcinoma cell line.Construction of primary hepatocellular carcinoma model in rat is divided into normal group, model Group and polydatin preventive group.Pathological examination showed that compared with the model group, polydatin control group of liver cancer incidence was significantly lower.Detecting the expression of microRNA-21 in liver tissue, the results showed that the Chenopodium glaucum The expression of microRNA-21 in the livers and the expression of the microRNA-21 target gene PTEN were significantly increased in the reteproliferative group.Presidin was significantly decreased in the hepatoma cell lines SMMC7721 and Hep G2 with a significant decrease in cell proliferation and invasion With the decrease of the expression of microRNA-21, the expression of PTEN is increased, suggesting that polydatin may inhibit the expression of microRNA-21 and inhibit the occurrence of hepatocellular carcinoma. The results provide a new theoretical basis for the prevention of primary liver cancer, However, its clinical efficacy needs further verification.