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目的:观察刺五加有效部位对MPP~+诱导损伤的PC12细胞LRRK 2蛋白表达的影响,探讨其神经保护的作用机制。方法:采用MPP~+作用于PC12细胞构建帕金森病的细胞模型,以刺五加有效部位进行给药干预;采用MTT法测细胞存活率;荧光定量PCR法检测细胞LRRK 2 mRNA的表达水平,Western blot法和免疫细胞化学法检测细胞LRRK 2蛋白的表达。结果:给药组与模型组比较,细胞存活率明显提高,差异有统计学意义(P<0.01);给药组与模型组比较,细胞中LRRK 2的蛋白表达水平明显降低,差异有统计学意义(P<0.01)。结论:刺五加有效部位对PD细胞模型具有保护作用,刺五加有效部位下调LRRK2蛋白的表达水平可能是其起到保护作用的机制之一。
Objective: To observe the effect of effective parts of Acanthopanax senticosus on the expression of LRRK 2 in PC12 cells induced by MPP ~ +, and to explore its mechanism of neuroprotection. Methods: The cell model of Parkinson’s disease was established by using MPP ~ + in PC12 cells, and the effective dose of Acanthopanax senticosus was administered to the cell model. Cell viability was measured by MTT assay. The expression of LRRK 2 mRNA was detected by real- Western blot and immunocytochemistry were used to detect the expression of LRRK 2 protein. Results: Compared with the model group, the cell survival rate was significantly increased in the treated group and the difference was statistically significant (P <0.01). Compared with the model group, the protein expression of LRRK 2 in the treated group was significantly decreased Significance (P <0.01). CONCLUSION: The effective site of Acanthopanax senticosus has a protective effect on PD cell model. The down-regulation of LRRK2 protein expression may be one of the protective mechanisms.