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目的观察高脂饮食对大鼠血管内皮功能和腹主动脉形态学的影响。方法选用健康SD雄性大鼠16只,体质量150～180g,随机分为正常对照组和高脂饮食组,每组8只。正常对照组用普通饲料喂养,高脂饮食组用高脂饲料喂养4周。观察高脂饮食对大鼠血浆血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)、血浆内皮素(endothelin,ET)和血清一氧化氮(nitrogen monoxide,NO)的影响,以及腹主动脉形态学变化。结果4周后高脂饮食组血浆血管紧张素Ⅱ、血浆内皮素与正常对照组相比明显升高(P<0.01),血清NO与正常对照组相比明显降低(P<0.01);光学显微镜、透射电镜下见高脂饮食组腹主动脉壁结构异常。结论高脂饮食可致大鼠血管内皮功能障碍和腹主动脉形态学改变。
Objective To observe the effect of high-fat diet on vascular endothelial function and morphology of abdominal aorta in rats. Methods Sixteen healthy SD male rats weighing 150-180 g were randomly divided into normal control group and high fat diet group, with 8 rats in each group. Normal control group fed with normal diet, high-fat diet group fed with high-fat diet for 4 weeks. To observe the effect of high-fat diet on plasma angiotensinⅡ (AngⅡ), endothelin (ET) and serum nitrogen monoxide (NO) in rats and the morphological changes of abdominal aorta. Results After 4 weeks, plasma levels of angiotensin Ⅱ and endothelin in high fat diet group were significantly higher than those in normal control group (P <0.01), serum NO was significantly lower than that in normal control group (P <0.01) , Under the transmission electron microscope see the high-fat diet abdominal aortic wall abnormalities. Conclusion High-fat diet can cause vascular endothelial dysfunction and morphological changes of abdominal aorta in rats.