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为明确镇稻2号、扬粳9538、徐稻3号及盐稻8号等4个粳稻品种驱避灰飞虱的原因,采用“H”型嗅觉仪测定灰飞虱对4个抗性品种稻株挥发物的行为反应,并通过GC-MS对挥发物的组分进行了定性和定量分析,测定其中4个组分对灰飞虱的生物活性。结果表明,灰飞虱明显偏好感虫对照武育粳3号稻株的挥发物和避开扬粳9538等抗性品种稻株的挥发物。GC-MS分析发现,抗虫稻株挥发物的组成与感虫对照武育粳3号之间存在着明显差异。感虫对照稻株的挥发物中有38种成分;与感虫稻株挥发物相比,徐稻3号、盐稻8号、扬粳9538、镇稻2号抗虫稻株挥发物中增加了未知20组分,且扬粳9538、镇稻2号抗虫稻株挥发物中还增加了橙花叔醇。此外,徐稻3号、扬粳9538、镇稻2号抗虫稻株挥发物中一些组分的相对含量较感虫对照中的有明显改变。生物活性测定表明,Z-法尼烯、橙花叔醇及雪松醇等3个组分对灰飞虱有明显的引诱作用。
In order to clarify the reasons for repelling Laodelphax striatellus in four japonica rice varieties, Zhenjiang 2, Yangjing 9538, Xuya 3 and Yanao 8, the effects of Laodelphax striatellus Sex breeds of rice volatiles behavioral response and volatile components by GC-MS were qualitative and quantitative analysis of four of which components of the biological activity of Laodelphax striatellus. The results showed that Laodelphax striatellus had a significant preference for the volatiles of the susceptible Wuliangjing 3 rice plant and the volatiles of the resistant rice variety Yangjing 9538. GC-MS analysis found that there was a significant difference between the composition of volatile compounds of insect-resistant rice plants and the susceptible control Wuyujing 3. There were 38 components in the volatiles of the susceptible control rice plants. Compared with the volatile compounds of the susceptible rice plants, the contents of volatile compounds in Xudian 3, Yanao 8, Yangjing 9538 and Zhendao 2 were increased Unknown 20 components, and Yangjing 9538, Zhenjiang 2 insect-resistant rice plant volatiles also increased nerolidol. In addition, the relative contents of some components in the volatile compounds of Xudao 3, Yangjing 9538 and Zhendao 2 were significantly changed compared with that of the control. Bioassay showed that three components, Z-farnesene, nerolidol and cedrol, could induce larval fecundity effectively.