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目的调查某医院阴沟肠杆菌临床分离株的耐药性和arm A基因分布情况,探索arm A阳性菌株与耐药性的关系及其分子分型特征。方法采用微量肉汤稀释法对51株临床分离的阴沟肠杆菌进行药敏试验;荧光定量PCR方法检测16S rRNA甲基化基因arm A;脉冲场凝胶电泳试验(PFGE)分析arm A阳性菌株间的亲缘关系。结果阴沟肠杆菌临床分离株对阿米卡星和庆大霉素的耐药率分别为39.2%和54.9%,arm A基因阳性率为23.5%,arm A阳性菌株对阿米卡星和庆大霉素均耐药;12株携带arm A基因菌株主要分为5型,无明显优势菌株。结论产16S rRNA甲基化酶arm A的阴沟肠杆菌菌株对氨基糖苷类药物耐药,应加强对该基因监测,合理指导临床抗生素应用。
Objective To investigate the drug resistance and arm A gene distribution of clinical isolates of Enterobacter cloacae in a hospital and to explore the relationship between arm A positive strains and drug resistance and their molecular typing characteristics. Methods 51 clinical isolates of Enterobacter cloacae were tested for susceptibility by using broth microdilution method. The methylation status of 16S rRNA gene arm A was detected by real-time fluorescence quantitative PCR (PFGE) The kinship. Results The clinical isolates of Enterobacter cloacae were 39.2% and 54.9% resistant to amikacin and gentamicin, respectively. The arm A gene positive rate was 23.5%. The arm A positive strains were resistant to amikacin and Qingda All of the 12 strains were resistant to arm A gene. The strains were divided into 5 types and no dominant strains were found. Conclusion Strains of Enterobacter cloacae producing 16S rRNA methylase arm A are resistant to aminoglycosides, and the monitoring of this gene should be strengthened to guide the clinical application of antibiotics.