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目的:探讨致敏大鼠抗原攻击后脑皮层和肺气道中干扰素-γ(IFN-γ)和白介素-4(IL-4)出现的相关性变化.方法:观察致敏大鼠吸入抗原诱导的支气管肺灌洗液(BALF)和肺组织切片炎症变化,用ELISA法测定BALF和脑皮层IFN-γ和IL-4水平变化.结果:抗原攻击组BALF中的炎症细胞数目明显高于对照未攻击组(P<0.05).地塞米松(DXM,0.5mg/kg,ip)明显减少BALF中的白细胞总数,几乎完全抑制嗜酸性粒细胞(EOS)和淋巴细胞的聚集,但增加中性粒细胞数目.抗原攻击组的组织学检查积分(EOS浸润、粘膜水肿和上皮损伤)也明显高于对照未攻击组(P<0.05).DXM(0.5 mg/kg,ip)减少支气管和细支气管的EOS数目,改善粘膜水肿和上皮损伤.致敏大鼠抗原攻击后,BALF中的IFN-γ水平降低伴随IL-4升高导致了IFN-γ/IL-4比例下降.与此同时,脑皮层匀浆中也出现相似的改变.DXM(0.5mg/kg,ip)能反转BALF和脑皮层匀浆中的IFN-γ/IL-4比例下降.结论:致敏大鼠抗原攻击后脑皮层和肺气道中的IFN-γ和IL-4出现相关性变化.
Objective: To investigate the correlation between the expression of interferon-γ (IFN-γ) and interleukin-4 (IL-4) in the cerebral cortex and pulmonary airway after sensitized rat antigen challenge.Methods: The sensitized rats were induced by inhaled antigen Bronchoalveolar lavage fluid (BALF) and lung tissue sections, and the levels of IFN-γ and IL-4 in BALF and cortex were determined by ELISA.Results: The number of inflammatory cells in BALF of the challenge group was significantly higher than that of the control group (DXM, 0.5 mg / kg, ip) significantly reduced the total number of leukocytes in BALF, almost completely inhibited the aggregation of eosinophils (EOS) and lymphocytes, but increased neutrophil (EOS infiltration, mucosal edema and epithelial damage) were also significantly higher than those in the control non-challenged group (P <0.05) .DXM (0.5 mg / kg, ip) decreased EOS in bronchus and bronchiole The number of mucosal edema and epithelial damage improved allergen rat antigen challenge, BALF IFN-γ levels decreased with increased IL-4 led to a decrease in the proportion of IFN-γ / IL-4 At the same time, the cerebral cortex uniform Similar changes also occurred in the pulp.DXM (0.5 mg / kg, ip) reversed the decrease in IFN-γ / IL-4 ratio in BALF and cortex homogenates. On: Changes associated antigen challenge in sensitized rat cerebral cortex and lung airways in IFN-γ and IL-4 appears.