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目的:探讨树突状细胞(DCs)活化的细胞毒T细胞(CTLs)联合5-氟尿嘧啶(5-FU)在裸鼠体内外对子宫颈癌的抑制作用。方法:MTT法检测DCs激活的CTLs联合5-FU在裸鼠体外对子宫颈癌细胞的抑制作用。裸鼠体内实验分为CTLs治疗组、5-FU治疗组、CTLs/5-FU治疗组及对照组,观察移植瘤成瘤率、潜伏期、瘤重及瘤体积。结果:治疗组在裸鼠体外都显著抑制子宫颈癌细胞生长,CTLs/5-FU治疗组最显著(P<0.01)。裸鼠体内实验中,治疗组的成瘤率、瘤体积、瘤重都显著降低,潜伏期延长,CTLs/5-FU治疗组最显著(P<0.01)。结论:联合应用DCs激活的CTLs及5-FU比单独应用CTLs或5-FU更有效。
Objective: To investigate the inhibitory effect of dendritic cells (DCs) activated cytotoxic T cells (CTLs) combined with 5-fluorouracil (5-FU) on cervical cancer in vitro and in vivo in nude mice. Methods: MTT assay was used to detect the inhibitory effect of DCs-activated CTLs combined with 5-FU on human cervical cancer cells in vitro. Nude mice in vivo experiments were divided into CTLs treatment group, 5-FU treatment group, CTLs / 5-FU treatment group and control group, the tumorigenic rate, incubation period, tumor weight and tumor volume were observed. Results: The treatment group significantly inhibited the growth of cervical cancer cells in vitro in nude mice. CTLs / 5-FU treatment group was the most significant (P <0.01). In nude mice, the tumor formation rate, tumor volume and tumor weight of the treatment group were significantly reduced, and the incubation period was prolonged. CTLs / 5-FU treatment group was the most significant (P <0.01). CONCLUSION: CTLs and 5-FU activated by DCs are more effective than CTLs or 5-FU alone.