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采用四唑盐比色法（ＭＴＴ）、３Ｈ－ＴｄＲ掺入试验、细胞大小测定的图象分析等方法，观察不同浓度牛血浆纤维粘连蛋白（ＦＭ）和人工合成肽段（ＲＧＤＳ，４０μｇ／ｍｌ）对体外培养的人牙髓细胞的影响。ＦＮ（２０、４０μｇ／ｍｌ）可促进牙髓成纤维细胞增殖、刺激细胞ＤＮＡ合成，ＦＮ（１０、８０μｇ／ｍｌ）、ＲＧＤＳ（４０μｇ／ｍｌ）和空白组对牙髓细胞无上述效应。４种浓度ＦＮ和ＲＧＤＳ（４０μｇ／ｍｌ）均使牙髓细胞处于伸展状态，支持细胞的粘附。说明ＦＮ可促进牙髓细胞增殖，并可通过整合素受体调节细胞与基质之间的联系。
The effects of different concentrations of bovine fibronectin (FM) and synthetic peptide (RGDS, 40μg / ml) were observed by MTT assay, 3H-TdR incorporation assay and image analysis of cell size. ) On human dental pulp cells cultured in vitro. FN (20,40μg / ml) could promote the proliferation of dental pulp fibroblasts, stimulate DNA synthesis, FN (10,80μg / ml), RGDS (40μg / ml) and blank group had no effect on dental pulp cells. Four concentrations of FN and RGDS (40μg / ml) all allowed the dental pulp cells to stretch and support cell adhesion. FN can promote dental pulp cell proliferation, and through the integrin receptors regulate cell-matrix connection.