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目的 建立反相高效液相色谱（ＲＰ－ＨＰＬＣ）法测定蛇床子药材中蛇床子素的含量的方法。方法 采用十八烷基硅烷键合硅胶柱Ｄｉａｍｏｎｓｉｌ（ＴＭ） 钻石Ｃ１８色谱柱，流动相为甲醇－水（７５∶２５），检测波长３２５ｎｍ。结果 蛇床子素在０．２８８～１．４４０μｇ范围内具有良好的线性关系，ｒ＝０．９９９０；本法精密度高，ＲＳＤ＝０．４３；重现性好，ＲＳＤ＝１．４１；回收率为９７．３８％ ，ＲＳＤ＝１．１１。结论 本方法可以精确测定蛇床子中蛇床子素的含量。
Objective To establish a reversed-phase high performance liquid chromatography (RP-HPLC) method for the determination of osthol in Cnidium monnieri. Methods Diamonsil(TM) diamond C18 column with octadecylsilane bonded silica column was used. The mobile phase was methanol-water(75∶25). The detection wavelength was 325nm. The results showed that the osthol had a good linearity in the range of 0.288～1.440μg, r=0.9990. The precision of this method was high, RSD=0.43, good reproducibility, RSD=1.41; The rate is 97.38% with RSD=1.11. Conclusion This method can accurately determine the content of osthole in Cnidium monnieri.