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以香菇（Ｌｅｎｔｉｎｕｓｅｄｏｄｅｓ）种内不同株一对亲和的单核菌丝（７４０２〈２〉和９１０１〈１２〉）为亲本，以碘乙酰胺失活７４０２〈２〉作为筛选标记，经过原生质体制备、ＰＥＧ融合及融合子再生等步骤，选育得融合子。融合子与双亲无拮抗性，在菌丝形态，核数目及可溶性蛋白质图谱、酯酶同工酶谱和过氧化物酶谱上均与双亲单核菌丝及担孢子杂交子有区别。将Ｋｎｏｗｌｔｏｎ等于１９８４年建立的一种分离高频再生衍生株的方法首次运用至食用菌中，使原生质体再生率提高２－３倍，为融合操作提供了方便。
A pair of affinity mononuclear mycelia (7402 <2> and 9101 <12>) of different strains of Lentinousodes were used as parents and Iodoacetamide inactivation 7402 <2> was used as a screening marker, and protoplasts were prepared , PEG fusion and fusion sub-regeneration steps, breeding fusants. There was no antagonism between the confluent and the parents, and there was a difference between the mycelial morphology, nuclear number and soluble protein profile, esterase isoenzyme pattern and peroxidase profile with the single parent hyphae and basidiomycete hybrids. The method of Knowlton et al. Established in 1984 to separate high frequency regenerated derivative strains was first applied to edible fungi to increase the regeneration rate of protoplasts by 2-3 times, which facilitated the fusion operation.