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鉴于以未经选择的实生苗为砧木进行的嫁接繁殖使同一橡胶无性系的不同植株之间在产量及生势方面依然存在相当程度的异质性,故人们迫切希望通过离体微繁途径克服这一难题。在离体微繁中,来自成龄植株及种子实生苗的茎节及茎尖材料均是可供利用的外植体来源。就橡胶无性系的离体微繁而言,所存在的最大问题是难以诱导出具有良好支撑固定作用的直根系,而且外植体对培养基的反应也相对较差。目前,通过离体微型嫁接已使这一问题得到有效改善。近年来,橡胶树体细胞胚胎发生的研究也引起人们越来越多的关注和重视,而且有些基因型已成功诱导体细胞胚胎发生及植株再生。在橡胶树中,至今还没有任何一种组培技术(包括微型扦插及体细胞胚胎发生等)被用于无性系的大规模繁殖。不过,随着研究水平的不断提高,橡胶树的离体微繁殖有望逐步成为现实。
Given that grafting with unselected seedlings as rootstocks still causes considerable heterogeneity in yield and vigor between different plants of the same rubber clones, there is a strong desire to overcome this problem by microprojection pathways ex vivo This problem. In vitro micropropagation, stem nodes and shoot tip materials from adult plant and seedling seedlings are all sources of explants available. In the case of in vitro micropropagation of rubber clones, the biggest problem is that it is difficult to induce straight roots with good support and fixation, and the explants respond relatively poorly to the medium. At present, this problem has been effectively improved through in vitro micro-grafting. In recent years, the research on somatic embryogenesis of rubber tree has aroused more and more attention and attention, and some genotypes have successfully induced somatic embryogenesis and plant regeneration. In the rubber tree, so far no technique of tissue culture (including micro-cutting and somatic embryogenesis, etc.) is used for large-scale breeding clones. However, with the continuous improvement of the research level, the in vitro micropropagation of rubber trees is expected to gradually become a reality.