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采用ＬｓＡＢ免疫组比方法，检测了连续２３例新鲜的食管鳞癌手术标本中癌组织Ｐ５３蛋白、增殖细胞核抗原（ＰＣＮＡ）和正常食管粘膜上皮Ｐ５３蛋白的表达。全组病例中癌组织Ｐ５３蛋白表达的阳性率为７３．９％，过度表达为４７．８％，同一病人配对的正常食管粘膜上皮Ｐ５３蛋白表达阳性率７８．３％，过度表达为４．３％。癌组织和正常粘膜上皮Ｐ５３蛋白表达的强度高峰位置呈右移现象，前者的强度高峰在位置上，后者则在±位置上。癌组织中ＰＣＮＡ呈１００％的表达，过度表达为５２．２％。食管鳞癌Ｐ５３蛋白过度表达与肿瘤分级有统计学上的显著性意义，但与肿瘤分期无统计学显著性意义，ＰＣＮＡ的过度表达与肿瘤分级、分期没有统计学上的显著性意义（Ｐ＞０．０５）．本研究提示，食管鳞癌之Ｐ５３蛋白表达高于其他肿瘤，其临床意义和应用价值可能更大，Ｐ５３蛋白和ＰＣＮＡ的表达没有相关关系（ｒｓ＝０．１２３２，Ｐ＞０．ｏ５）。
The expression of P53 protein, proliferating cell nuclear antigen (PCNA) and P53 protein in normal esophageal mucosal epithelium in 23 consecutive specimens of esophageal squamous cell carcinoma were detected by LsAB immunohistochemistry. The positive rate of P53 protein expression in cancer tissue was 73.9% in the whole group and overexpression was 47.8%. The positive rate of P53 protein expression in the normal esophageal mucosal epithelium paired with the same patient was 78.3%. The overexpression was 4.3. %. The peak intensity of P53 protein expression in cancerous tissues and normal mucosal epithelium shifted to the right. The peak intensity of the former was at the position and the latter was at the ± position. The expression of PCNA in cancer tissue was 100%, and overexpression was 52.2%. The overexpression of P53 protein and tumor grading in esophageal squamous cell carcinoma were statistically significant, but it was not statistically significant with tumor stage. There was no statistically significant difference between PCNA overexpression and tumor grading and staging. 0.05). This study suggests that the expression of P53 protein in esophageal squamous cell carcinoma is higher than that of other tumors, and its clinical significance and application value may be even greater. There is no correlation between the expression of P53 protein and PCNA (rs=0.1232, P>0.o5).