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目的 提供改良的制作大鼠局部缺血性脑损伤模型的方法。方法 雄性Wistar大鼠 44只 ,体重 1 60～1 80g,随机分为空白对照组 (n =6)、假手术组 (n =1 5)、缺血损伤再灌注组 (n =2 3) ,35只用于脑的大体和组织学研究 ,9只用于核磁共振T2 WI成像研究。颈部正中切口 ,采用从颈内动脉插入线栓的造模方法 ,用 5/ 0尼龙线栓造成大脑中动脉血供阻断 (MCAO) 2h。缺血损伤再灌注组的再灌时间点为 1d(n =8)、2d(n=5)、3d(n =5)、7d(n =5)。采用大体和组织学观察方法 ,研究脑组织梗死灶及血管的变化。采用核磁共振T2 WI成像技术 ,检测大脑高信号强度区信号的变化。结果 空白对照组、假手术组未见梗死灶 ,缺血损伤再灌注组有梗死灶的形成 ,7d时的梗死灶体积为 (1 1 8.65± 43 .61 )mm3。结论 改良的线栓阻断大脑中动脉制作缺血性脑损伤模型的方法稳定可靠 ,手术过程简化 ,容易操作。
Objective To provide an improved method of making a rat model of ischemic brain injury. Methods Forty - four male Wistar rats weighing 160 to 180 g were randomly divided into blank control group (n = 6), sham operation group (n = 15), ischemic injury and reperfusion group (n = 23) 35 were used for gross and histological brain studies, and 9 for magnetic resonance T2WI imaging studies. The middle incision of the neck was occluded with a middle cerebral artery occlusion (MCAO) with a 5/0 nylon plug for 2 h using a modeling method of inserting a thread plug from the internal carotid artery. The reperfusion time point of ischemic injury reperfusion group was 1d (n = 8), 2d (n = 5), 3d (n = 5) and 7d (n = 5). The gross and histological methods were used to study the changes of cerebral infarction and blood vessels. T2 WI imaging was used to detect the signal changes in the high signal intensity area of the brain. Results No infarction was found in the blank control group and the sham operation group. The infarct size was formed in the ischemic reperfusion group. The volume of infarct volume at 7 days was (1 1 8.65 ± 43.61) mm 3. Conclusion The improved method of occlusion of the middle cerebral artery by the occlusion of the middle cerebral artery with ischemic brain injury model is stable and reliable, the operation process is simplified and easy to operate.