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目的研究低温治疗大鼠创伤性脑损伤后对脑水含量、Na+含量及白细胞介素-1β(IL-1β)的影响,以探讨低温的治疗作用及可能机制。方法采用Feeney打击模型,分别进行全身低温、全脑低温或局灶低温治疗,治疗结束后检测伤灶脑组织含水量、Na+含量、IL-1β含量、IL-1β蛋白表达及IL-1βmRNA表达。结果局灶低温组及全身低温组在脑水含量、Na+含量、IL-1β含量、IL-1β蛋白表达、IL-1βmRNA表达方面低于脑外伤模型组(P<0.05),且局灶低温组低于全身低温组(P<0.05);而全脑低温组在上述方面高于脑外伤模型组(P<0.05)。结论局灶低温及全身低温治疗能明显减轻脑水肿,且局灶低温效果更佳;全脑低温治疗加重脑水肿;其机制之一可能是通过抑制或促进炎性因子IL-1β表达而起作用。
Objective To investigate the effects of hypothermia on brain water content, Na + content and interleukin-1β (IL-1β) after traumatic brain injury in rats, in order to investigate the therapeutic effect and possible mechanism of hypothermia. Methods The model of Feeney attack was used to treat systemic hypothermia, whole brain hypothermia and focal hypothermia respectively. After the treatment, the content of water, Na +, IL-1β, IL-1β and IL-1βmRNA were detected. Results The levels of brain water, Na +, IL-1β, IL-1β and IL-1βmRNA in focal hypothermia group and general hypothermia group were lower than those in traumatic brain injury model group (P <0.05) Lower than the whole body hypothermia group (P <0.05), while in the whole brain hypothermia group was higher than that of the traumatic brain injury model group (P <0.05). Conclusions Focal hypothermia and systemic hypothermia can significantly reduce cerebral edema, and the effect of focal hypothermia is better. Whole brain hypothermia treatment aggravates cerebral edema. One of the mechanisms may be through inhibiting or promoting the expression of inflammatory factor IL-1β .