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目的通过建立小鼠肝转移模型探讨肌醇六磷酸(IP6)和肌醇(Ins)联合应用对肿瘤转移血管生成因子的影响。方法脾膜注射方法建立结直肠癌肝转移模型后,将48只BALB/c小鼠按体质量随机区组为IP6组、Ins组、IP6+Ins组、阴性对照组,每组12只。术后第2日,实验组小鼠给予剂量为80 mg/kg bw的IP6和Ins灌胃,合用组体积以1:1比例混合,对照组以等体积的生理盐水代替,每天1次。连续给药20d后处死小鼠,观察瘤重和肝转移抑制率;采用RT-PCR和免疫组织化学方法检测小鼠血管内皮生长因子(VEGF);碱性成纤维生长因子(b FGF);转化生长因子(TGF-β)m RNA和蛋白的表达。结果与对照组相比,IP6、Ins、IP6+Ins组小鼠的瘤重和肝转移抑制率都降低(P<0.05);VEGF、b FGF、TGF-β的m RNA和蛋白的表达水平降低(P<0.05),并且合用组的效果优于单独使用,P<0.05。结论 IP6和Ins联合应用通过阻滞肿瘤血管生成因子VEGF、b FGF、TGF-β的表达明显抑制肿瘤生长和肝转移的发生,并且合用的效果优于单独使用。
OBJECTIVE: To investigate the effects of IP6 and Ins on tumor metastasis of angiogenesis by establishing mouse liver metastasis model. Methods After the model of liver metastasis of colorectal cancer was established by the method of spleen membrane injection, 48 BALB / c mice were randomly divided into IP6 group, Ins group, IP6 + Ins group and negative control group. On the second day after operation, the mice in the experimental group were administered with IP6 and Ins at a dose of 80 mg / kg bw, and the volume of the combined group was mixed in a ratio of 1: 1. The control group was replaced with an equal volume of physiological saline once a day. Mice were sacrificed after continuous administration for 20 days to observe the inhibition of tumor weight and hepatic metastasis. The levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were detected by RT-PCR and immunohistochemistry. Growth factor (TGF-β) m RNA and protein expression. Results Compared with the control group, the inhibition rates of tumor weight and hepatic metastasis of IP6, Ins and IP6 + Ins groups were all decreased (P <0.05), and the expression of m RNA and protein of VEGF, b FGF and TGF-β were decreased (P <0.05), and the combination group was better than single use, P <0.05. Conclusion The combination of IP6 and Ins can inhibit tumor growth and liver metastasis by blocking the expression of tumor angiogenic factors VEGF, b FGF and TGF-β, and the combined effect is better than single use.