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目的:研究活血化瘀中药抗小鼠术后病理性腹腔粘连的作用。方法:昆明小鼠(清洁级)120只,造模后随机分成5组,A组(模型组)、B组(丹参酮ⅡA磺酸钠组)、C组(盐酸川芎嗪组)、D组(尿激酶组)、E组(阿司匹林组),术后第1d、2d腹腔给药,饲养14d,于术后第15d处死小鼠,进行腹膜粘连评分及病理HE染色、免疫组化SABC法及Masson染色观察,并统计分析。结果:腹膜Nair粘连评分提示A组粘连中位数为Ⅲ级,B、C、D组则为Ⅱ级,B、C、D与A组两两比较均有统计学意义(P=0.0271,P=0.0021,P=0.0058);HE染色提示C与A、B、E组两两比较均有统计学意义(P<0.0001,P=0.0479,P=0.0319);Masson染色提示C与A、B、D、E组两两比较均有统计学意义(P<0.05);Colla鄄genⅠ免疫组化提示C、D分别与A、B、C、E组两两比较均有统计学意义(P<0.05)。结论:药物抗粘连效果为C≥D>B>E≥A,丹参酮ⅡA、川芎嗪及尿激酶均有预防并减轻腹腔粘连作用,尤以川芎嗪与尿激酶效果最佳。
Objective: To study the effect of traditional Chinese medicine for promoting blood circulation and removing blood stasis on postoperative pathological abdominal adhesions in mice. METHODS: A total of 120 Kunming mice (Clean grade) were randomly divided into 5 groups: A group (model group), B group (Tanshinone IIA sodium sulfonate group), C group (Ligustrazine hydrochloride group), and D group ( Urokinase group) and E group (aspirin group) were intraperitoneally administered on the 1st and 2nd postoperative day and fed for 14 days. The mice were sacrificed on the 15th day after operation. The peritoneal adhesion score and pathological HE staining, immunohistochemical SABC method and Masson were performed. Staining observations and statistical analysis. RESULTS: The peritoneal Nair adhesion scores showed that the median adhesion was grade III in group A, grade II in group B, C, and D, and statistically significant in groups B, C, D, and A (P=0.0271, P). = 0.0021, P = 0.0058); HE staining indicated that there was a statistically significant difference between C and A, B and E groups (P<0.0001, P=0.0479, P=0.0319); Masson staining suggested that C and A, B, There was a statistically significant difference between D and E groups (P<0.05). The immunohistochemistry of Colla鄄gen I suggested that both C, D and A, B, C and E groups were statistically significant (P<0.05). ). Conclusion: The anti-adhesive effect of the drug is C≥D>B>E≥A. Tanshinone IIA, tetramethylpyrazine and urokinase all have the effect of preventing and relieving abdominal adhesions, especially the effects of ligustrazine and urokinase.