论文部分内容阅读
目的:探讨克拉维酸对抗大鼠脑缺血的作用及其机制。方法:实验分8组:空白对照组,假手术组,溶剂对照组,克拉维酸对照组,全脑缺血-再灌注损伤组,以及克拉维酸低、中、高剂量保护组。采用四血管闭塞法建立动物全脑缺血模型;术后3 d通过硫堇尼氏体染色观察海马CA1区细胞形态;术后1 d通过RT-PCR及ELISA分析海马组织TNF-α、IL-1β及IL-6mRNA及蛋白含量。结果:克拉维酸可减少全脑缺血引起的大鼠海马CA1区锥体神经元迟发性神经元死亡,可降低缺血组织中炎症因子TNF-α、IL-1β及IL-6 mRNA及蛋白表达水平。结论:克拉维酸可对抗脑缺血,发挥神经保护作用,其保护作用与抑制炎症因子表达有关。
Objective: To investigate the effect of clavulanic acid on cerebral ischemia in rats and its mechanism. Methods: The experiment was divided into 8 groups: blank control group, sham operation group, solvent control group, clavulanic acid control group, global cerebral ischemia-reperfusion injury group and clavulanic acid low, medium and high dose protection group. The model of global cerebral ischemia was established by four vessel occlusion. The morphology of hippocampal CA1 region was observed by thionine staining 3 days after operation. The expression of TNF-α and IL- 1β and IL-6 mRNA and protein content. Results: Clavulanic acid could reduce the delayed neuronal death in hippocampal CA1 pyramidal neurons induced by global cerebral ischemia in rats, reduce the expression of inflammatory cytokines TNF-α, IL-1β and IL-6 mRNA Protein expression levels. Conclusion: Clavulanic acid can antagonize cerebral ischemia and exert neuroprotective effect. Its protective effect is related to inhibiting the expression of inflammatory cytokines.