目的探讨白藜芦醇(resveratrol)对肝脏损伤的保护作用。方法 50只小鼠随机分成5组,每组10只,分别为:正常对照组、模型组、白藜芦醇低剂量组、白藜芦醇中剂量组、白藜芦醇高剂量组。实验前两周,白藜芦醇组各灌服相应的药物一日0.1 ml/10gBW。正常组和模型组则灌服0.5%羧甲基纤维素溶液一日0.1 ml/10gBW。第14天灌服结束后1小时,除正常组外各组动物均从尾静脉注射刀豆蛋白溶液一日0.05 ml/10gBW,正常对照组动物则尾静脉注射生理盐水一日0.05 ml/10gBW。测定ALT、AST;取肝右叶置于4%多聚甲醛4℃下固定,用于HE染色。结果肝脏肉眼观察及HE染色后病理观察均提示模型组肝脏损伤明显重于白藜芦醇组,且模型组血清ALT、AST水平与对照组相比升高,差别具有显著统计学意义(P<0.01)。白藜芦醇高、中、低剂量组血清ALT、AST水平均较模型组下降,差别具有显著统计学意义(P<0.01)。白藜芦醇各剂量组间的差异也无统计学意义。与正常组相比,不同浓度白藜芦醇治疗组中炎症因子IL-2、IL-6、TNF-α表达量增加,各浓度间无明显差异。与模型组相比,各浓度组中炎症因子表达量明显下降。结论白藜芦醇对刀豆蛋白诱导的急性肝损伤有明显的保护作用。 Objective To investigate the protective effect of resveratrol on liver injury. Methods Fifty mice were randomly divided into five groups (n = 10): normal control group, model group, low dose resveratrol group, middle dose resveratrol group and high dose resveratrol group. Two weeks before the experiment, the resveratrol group fed the corresponding drug 0.1 ml / 10gBW on the 1st. The normal group and the model group were fed 0.5% carboxymethyl cellulose solution on the 1st day 0.1 ml / 10gBW. On the 14th day after the end of gavage, animals in each group were injected with concanavalin solution 0.05 ml / 10 g BW on the 1st day and animals in the normal control group were given saline 0.05 ml / 10 g BW on the 1st day. ALT, AST were measured; the right lobe of the liver was fixed in 4% paraformaldehyde at 4 ° C for HE staining. Results The liver and pathological changes of liver showed that the liver injury of the model group was more severe than that of the resveratrol group, and the serum ALT and AST levels in the model group were significantly higher than those in the control group (P < 0.01). Serum levels of ALT and AST in high, medium and low doses of resveratrol were significantly lower than those in model group, with significant difference (P <0.01). Resveratrol dose groups between the differences were not statistically significant. Compared with the normal group, the expression levels of IL-2, IL-6 and TNF-αin the resveratrol treated groups were increased, but there was no significant difference among the concentrations. Compared with the model group, the concentration of inflammatory cytokines in each group decreased significantly. Conclusions Resveratrol has obvious protective effect on the acute liver injury induced by concanavalin.