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[Objective] To research on the isolation,identification and determination of verbascoside in Callicarpa longissima( Hemsl.)Merr. [Methods]Verbascoside was extracted by 95% ethanol,isolated and purified by column chromatography and PHPLC from the stem and branch of Callicarpa longissima( Hemsl.) Merr. A HPLC method was set up to determine the content of verbascoside in Callicarpa longissima( Hemsl.) Merr,using ECOSIL ODS- EXTEND column( 4. 6 mm × 250 mm,5 μm),and acetonitrile-0. 2%- phosphoric acid water solution as the mobile phase,the UV detection wavelength was at 334 nm,with the flow rate of 1. 0 m L / min at room temperature.[Results] A good linear was obtained in the range of 0. 051 9 μg- 0. 829 6 μg and the average recovery was 99. 13%( RSD = 1. 80%,n = 9).[Conclusions]Verbascoside was isolated from the plant for the first time. This detection method was simple,feasible and repeatable,and was reliable for the quality control of C. longissima.
[Objective] To research on the isolation, identification and determination of verbascoside in Callicarpa longissima (Hemsl.) Merr. [Methods] Verbascoside was extracted by 95% ethanol, isolated and purified by column chromatography and PHPLC from the stem and branch of Callicarpa longissima (Hemsl.) Merr. A HPLC method was set up to determine the content of verbascoside in Callicarpa longissima (Hemsl.) Merr, using ECOSIL ODS- EXTEND column (4.6 mm × 250 mm, 5 μm) . 2% - phosphoric acid water solution as the mobile phase, the UV detection wavelength was at 334 nm with the flow rate of 1. 0 m L / min at room temperature. [Results] A good linear was obtained in the range of 0. 051 9 μg- 0. 829 6 μg and the average recovery was 99. 13% (RSD = 1.80%, n = 9). [Conclusions] Verbascoside was isolated from the plant for the first time. This detection method was simple, feasible and repeatable, and was reliable for the quality control of C. longissima.