论文部分内容阅读
目的探讨树突状细胞(Dendritic cell,DC)在非梗阻性无精子症患者睾丸中的免疫表型及其分子机制。方法采用免疫组织化学染色法检测10例正常人及25例无精子症患者睾丸中CD3,CD1a,CD11c,CD123,DC-SIGN(DC-specific ICAM-grabbing non-integrin),CD83,HLA-DR表达水平,并进行体视学分析。结果正常组及无精子症组均有CD1a、CD11c、DC-SIGN、HLA-DR表达,并主要分布于睾丸间质。无精子症组CD1a、CD11c、DCSIGN、HLA-DR表达水平较正常组显著增高,差异有显著性(P<0.05)。正常组无CD123及CD83表达,无精子症组CD123、CD83高表达。结论非梗阻性无精子症患者睾丸中CD1a+朗格汉斯细胞(Langerhans cell,LC)、DCSIGN+间质型DC(Stromal DC)、CD123+浆细胞样DC(Plasmacytoid DC,pDC)及CD11e+髓样DC(Myeloid DC,mDC)表达水平显著增高并趋向分化成熟,提示DC在无精子症发生发展过程中可能起重要作用。
Objective To investigate the immunophenotype of dendritic cells (DCs) in testis of non-obstructive azoospermia and its molecular mechanisms. Methods Immunohistochemical staining was used to detect the expression of CD3, CD1a, CD11c, CD123, DC-specific ICAM-grabbing non-integrin, CD83 and HLA-DR in 10 normal subjects and 25 patients with azoospermia Level, and stereological analysis. Results The expressions of CD1a, CD11c, DC-SIGN and HLA-DR in normal group and azoospermia group were mainly distributed in testis interstitium. The expression of CD1a, CD11c, DCSIGN and HLA-DR in azoospermia group was significantly higher than that in the normal group (P <0.05). No normal group CD123 and CD83 expression, no sperm group CD123, CD83 high expression. Conclusion CD1a + Langerhans cells (LC), DCSIGN + Stromal DCs, CD123 + plasmacytoid DCs (pDC) and CD11e + myeloid DCs in non-obstructive azoospermia patients Myeloid DC, mDC) were significantly increased and tended to differentiate, suggesting that DC may play an important role in the development of azoospermia.