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在昆明地区栽培的香石竹上发现了香石竹斑驳病毒,人工接种可局部侵染苋色藜、墙生藜、千日红及番杏,可系统侵染昆诺阿藜和美国石竹,分别造成褪绿斑、斑驳或坏死斑。经二轮PEG(分子量6000)沉淀,病毒提纯量约220mg/kg鲜组织。电镜观察病毒颗粒直径为28nm,等轴对称。提纯物紫外扫描呈典型病毒核蛋白吸收峰,A260/A280值为1.56。提纯病毒经4次免疫家兔,所得抗血清经试管沉淀测定效价为1∶4096,琼脂糖双扩散效价为1∶1024。
Carnation mottle virus was found on the carnation cultivated in Kunming. Artificial inoculation could infect parthenogenetic quinoa, wall-wall quinoa, aurora and apricot apricot, which could infect Quinuona trifolia and American carnation respectively, resulting in Faded spots, mottled or necrotic spots. After two rounds of PEG (molecular weight of 6000) precipitation, virus purification amount of about 220mg / kg fresh tissue. Electron microscopy virus particle diameter of 28nm, equiaxed symmetry. UV scanning showed a typical peak of the virus nucleoprotein, A260 / A280 value of 1.56. The purified virus was immunized rabbits 4 times, the resulting antiserum by tube precipitation assay titer 1:4096, agarose double diffusion titer 1:1024.