目的:探讨使用透明带结合后的精子行ICSI,是否可改善ICSI治疗结局。方法:选择上一周期IVF完全不受精者或男方为少、弱、畸形精子症而接受ICSI治疗的不育患者,共收录106对不孕夫妇,将其随机分成常规ICSI对照组和利用未成熟卵透明带上结合的精子进行ICSI的试验组,每组53例,分析比较ICSI结局。结果:试验组的卵裂率、优质胚胎率、可使用胚胎率均显著高于对照组,差异有统计学意义(P<0.05)。虽然临床妊娠率试验组(36%,19/53)与对照组(49%,26/53)组间无统计学差异(P>0.05),但试验组种植率(29%)、多胎率(35.71%,10/26)明显高于对照组(17%、10.53%,2/19)(P<0.01)。结论:通过利用患者自体ZP结合的精子进行ICSI注射,能获取高质量胚胎和高种植率,这种改良的ZP结合ICSI方法或许能为今后ICSI治疗提供一个新的思路。 OBJECTIVE: To investigate whether ICSI with zona pellucida combined with sperm can improve ICSI treatment outcome. Methods: A total of 106 pairs of infertile women were enrolled in this study. One hundred and sixty infertile couples were enrolled in this study. The infertile couples were enrolled in this study. The infertile couples were randomly divided into routine ICSI control group and immature Ovarian zona pellucida combined with sperm ICSI experimental group, each group of 53 cases, analysis and comparison of ICSI outcomes. Results: The cleavage rate, the rate of high quality embryos and the rate of embryos available in the test group were significantly higher than those in the control group (P <0.05). Although clinical pregnancy rate was not significantly different between the experimental group (36%, 19/53) and the control group (49%, 26/53) (P> 0.05), the implantation rate in the experimental group was 29% 35.71%, 10/26) was significantly higher than the control group (17%, 10.53%, 2/19) (P <0.01). CONCLUSIONS: High-quality embryos and high implantation rates can be obtained by using the patient’s own ZP-bound sperm for ICSI injection. This improved ZP combined with ICSI approach may provide a new insight into ICSI therapy in the future.