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目的探讨适合于城区不同生境的最佳蚊媒密度监测方法,了解城区内西尼罗病毒(WNV)潜在蚊媒种群构成、季节消长及带毒情况,为制定、评价和调整蚊虫控制措施提供依据,并为WNV的传入与出现进行必要的理论和技术储备。方法通过比较3种不同成蚊密度监测方法对不同生境的蚊虫诱捕效果,筛选最佳的监测方法;采用该方法对城区内不同生境的WNV潜在蚊媒进行调查并进行蚊虫采集;利用Vec Test抗原分析试剂盒和Ramp分析系统2种快速检测方法对采自城区内的蚊虫进行WNV检测。结果 3种监测方法对不同生境的蚊虫诱捕效果存在显著差异,密度高低顺序依次为:人帐诱法[4.167只/(帐·h)]>CO2诱蚊灯法[2.214只/(灯·h)]>诱蚊灯法[0.577只/(灯·h)],CO2诱蚊灯法诱捕种类多于其他2种方法;城区内共监测到3种西尼罗病毒潜在蚊媒,分别为淡色库蚊(占71.28%)、白纹伊蚊(占27.21%)和三带喙库蚊(占1.51%)。全年平均蚊密度为0.853只/(灯·h),蚊虫高峰期发生在7月上旬,为2.196只/(灯·h);对连续2年诱捕的1 515只蚊虫进行WNV检测,均为阴性。结论 CO2诱蚊灯法可作为蚊媒传染病发生时期进行传播媒介的调查及需要采集大量雌蚊进行相关病原体检测时捕获蚊虫的方法;明确了城区内不同生境的WNV潜在蚊媒种群构成及季节消长情况;未检测到不同生境中的蚊虫携带WNV。
Objective To explore the best mosquito density monitoring method suitable for different habitats in urban areas, to understand the composition, seasonal fluctuation and poisoning of potential mosquito vectors in West Nile virus (WNV) in urban areas, and to provide the basis for the formulation, evaluation and adjustment of mosquito control measures , And make the necessary theoretical and technical reserves for the introduction and emergence of WNV. Methods By comparing the mosquito trapping effects of three different adult mosquitoes in different habitats, the best screening method was screened. Potential mosquitoes of WNV in different habitats in urban areas were investigated and collected by mosquitoes. Vec Test antigen Analysis kits and Ramp analysis system Two rapid detection methods for mosquitoes collected from urban areas for WNV detection. Results There were significant differences in mosquito trapping effects among different habitats in the three monitoring methods. The order of the densities was as follows: 4.177 / (g · h)]> CO2 larvae method [2.214 / (lamp · h )]> The mosquito lamp method [0.577 / (lamp · h)], the CO2 trap lamp method captured more species than the other two methods; three kinds of potential West Nile virus mosquito vectors were detected in the urban area, which were light colors Culex (account for 71.28%), Aedes albopictus (27.21%) and Culex tritaeniorhynchus (1.51%). The average annual mosquito density was 0.853 / (lamp · h). The mosquito peak was 2.196 / (lamp · h) in the first ten days of July. The WNV detection of 1 515 mosquitoes trapped in two consecutive years were negative. CONCLUSION CO2-based mosquito lamp method can be used as a vector to detect mosquito-borne diseases and a method to capture mosquitoes when collecting a large number of female mosquitoes for pathogen-related detection. The potential mosquito population structure and seasons of WNV in different habitats in urban areas are clarified Growth and decline; no detection of mosquitoes carrying WNV in different habitats.