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食物过敏是由于接触特定过敏原引起的异常免疫反应。西红柿营养价值丰富并且可作为天然抗氧化剂,已被人们熟识并广泛食用的蔬菜。为了对西红柿中的内源性致敏蛋白有全面的认识和了解,本工作利用全序列比对的方法比较了西红柿蛋白与已知致敏原之间的序列相似性,获得了西红柿潜在致敏蛋白序列,并分析及描述了它们的保守结构域、分子作用功能、系统发育情况以及在染色体的位置。结果表明:共鉴定120个西红柿内源性致敏蛋白,其中包括9个已被数据库收录的西红柿致敏原;保守结构域分析显示它们分别属于43个蛋白家族,主要分布于Gelsolin、Chloroa_b-bind和Thaumatin等家族,其中的HATPase_c结构域未在已知致敏蛋白中发现;Chloroa_b-bind和Thaumatin家族蛋白在绿色植物较特有,将这2个家族蛋白进行系统发育分析则分别揭示了西红柿的遗传多样性;而且预测的致敏蛋白在西红柿染色体上有成簇状分布的趋势。
Food allergies result from an abnormal immune response caused by exposure to specific allergens. Tomatoes are rich in nutritional value and can be used as natural antioxidants, has been well-known and widely consumed vegetables. In order to fully understand and understand the endogenous sensitized proteins in tomato, this work compared the sequence similarity between tomato proteins and the known allergens by the method of full sequence alignment, and obtained the potential sensitization of tomato Protein sequences, and analyzed and described their conserved domains, molecular function, phylogenetic and chromosomal location. The results showed that 120 tomato endogenous sensitized proteins were identified, including nine tomato allergens which were included in the database. The conserved domain analysis showed that they belong to 43 protein families, mainly distributed in Gelsolin, Chloroa_b-bind And Thaumatin. Among them, the HATPase_c domain was not found in the known allergens. Chloroa_b-bind and Thaumatin family proteins were found in green plants. The phylogenetic analysis of these two families revealed the genetic variation of tomato Diversity; and predict the allergenic protein clusters in tomato chromosomes trend.