Mianmin地区位于巴布亚新几内亚的西北部,海拔100—1200米,人口流动频繁,是疟疾和班氏丝虫病的流行区。作者在1986年3—5月间,在不同的海拔高度,设6个点采集蚊,以时间18:00—6:00为一个“采集夜”。捕获的蚊经麻醉鉴定后分别处理。按蚊用硅胶干燥保存,以ELISA法,经单克隆抗体2A10和NSV3分别检测恶性疟原虫和间日疟原虫的环子孢子抗原(CS);库蚊保存于乙醇中,解剖检查幼丝虫感染情况。 Located in the north-west of Papua New Guinea, the Mianmin area is 100-1200 meters above sea level with frequent population movements and is a prevalent area for malaria and Bancroftian filariasis. From March to May 1986, at different altitudes, the author collected 6 mosquitoes at a time and took a collection night at 18: 00-6: 00. Captured mosquito after anesthesia identification were treated. Anopheles mosquitoes were dried and stored in silica gel. The circumsporozoite antigen (CS) of Plasmodium falciparum and Plasmodium vivax was detected by ELISA with monoclonal antibodies 2A10 and NSV3 respectively. Culex pipiens pallens were stored in ethanol and dissected for infection of young filarial worms Happening.